Human IL-18 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-18 Protein is expressed from E.coli without tag.
It contains Tyr37-Asp193 [Accession | Q14116].

分子量大小(Molecular Weight)

The protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human IL-18 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human IL-18 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human IL-18 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test).

SPR Data

Human IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).

Cell Based Assay

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 1-5 ng/mL (QC Test).

背景(Background)

Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.

分子别名(Synonyms)

IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1

文献(References)

(1)Yamada T, Aoyama-Ishikawa M, Yamashita H, Fujiwara M, Usami M, Ueda T, Terashima M, Kohama K, Nakao A, Kotani J. IL-18 production and IL18 promoter polymorphisms correlate with mortality in ICU patients. In Vivo. 2014 May-Jun;28(3):391-6. PMID: 24815843.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.