货号价格

SLA-HM1MF-100μg / 询价

SLA-HM1MF-500μg / 询价

SLA-HM1MF-500μgx2 / 询价

Human SLAMF1/SLAM/CD150 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human SLAMF1/SLAM/CD150 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala21-Pro237.[Accession | Q13291-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 25.4 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human SLAMF1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human SLAMF1 is greater than 95% as determined by SEC-HPLC.

背景(Background)

The signaling lymphocytic activation molecule (SLAM) family is comprised of nine distinct receptors (SLAMF1 through SLAMF9) that are expressed on hematopoietic cells.The SLAM family receptor function is largely controlled via SLAM associated protein (SAP) family adaptors. The SAP family adaptors consist of SAP, Ewing sarcoma associated transcript (EAT)-2, and EAT-2-related transducer (ERT).

分子别名(Synonyms)

CDw150; IPO-3; CD150; SLAMF1; SLAM

文献(References)

(1) Ma C, Wang N, Detre C, Wang G, O'Keeffe M, Terhorst C. Receptor signaling lymphocyte-activation molecule family 1 (Slamf1) regulates membrane fusion and NADPH oxidase 2 (NOX2) activity by recruiting a Beclin-1/Vps34/ultraviolet radiation resistance-associated gene (UVRAG) complex. J Biol Chem. 2012 May 25;287(22):18359-65. doi: 10.1074/jbc.M112.367060. Epub 2012 Apr 9. PMID: 22493499; PMCID: PMC3365751.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.