货号价格

NOG-HM001-100μg / 询价

NOG-HM001-500μg / 询价

NOG-HM001-500μgx2 / 询价

Human Noggin Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Noggin Protein is expressed from CHO without tag.
It contains Gln28-Cys232 [Accession | Q13253].

分子量大小(Molecular Weight)

The protein has a predicted MW of 23.05 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

内毒素(Endotoxin)

Less than 0.05EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Noggin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Noggin is greater than 95% as determined by SEC-HPLC.

Cell Based Assay

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP‑4.

背景(Background)

Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt.

分子别名(Synonyms)

Noggin; NOG; SYM1; SYNS1

文献(References)

(1) Sun Z, Gao X, Zabkiewicz C, Ruge F, Xie M, Cai S, Sun PH, Griffiths P, Pugh MR, Ji J, Jiang WG, Ye L. Noggin is associated with a poor prognosis of gastric cancer by promoting the proliferation of gastric cancer cells via the upregulation of EGFR. Int J Oncol. 2020 Sep;57(3):813-824. doi: 10.3892/ijo.2020.5081. Epub 2020 Jun 16. PMID: 32705152.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.