货号价格

NEC-HM404-100μg / 询价

NEC-HM404-500μg / 询价

NEC-HM404-1mg / 询价

Human Nectin-4 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Nectin-4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly32-Val351.[Accession | Q96NY8]

分子量大小(Molecular Weight)

The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Nectin-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Nectin-4 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Nectin-4 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin4 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.

背景(Background)

Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.

分子别名(Synonyms)

EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4

文献(References)

(1)Challita-Eid P M , Satpayev D , Yang P , et al. Enfortumab Vedotin Antibody-Drug Conjugate Targeting Nectin-4 is a Highly Potent Therapeutic Agent in Multiple Preclinical Cancer Models[J]. Cancer Research, 2016:0008-5472.CAN-15-1313.

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