KLK-CM102 | 价格

KLK-CM102-100μg / 询价

KLK-CM102-500μg / 询价

KLK-CM102-500μgx2 / 询价

Rhesus macaque Kallikrein 2/KLK2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Rhesus macaque Kallikrein 2/KLK2 Protein is expressed from HEK293 with His tag at the C-terminus [Accession | XP_014980161.3].

分子量大小(Molecular Weight)

The protein has a predicted MW of 27.63 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under Non-reduced condition based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20mM Tris, 150mM NaCl, 8% trehalose, 0.05% Brij-35, 5mM Benzamidine (pH 7.5).

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Rhesus macaque Kallikrein 2 on Bis-Tris PAGE under Non-reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Rhesus macaque Kallikrein 2 is greater than 95% as determined by SEC-HPLC.
ELISA Data
Immobilized Rhesus macaque Kallikrein 2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for h11B6, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. (QC Test)
 
Immobilized Rhesus macaque Kallikrein 2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Pasritamig with the EC50 of 12.5ng/ml determined by ELISA.
SPR Data
h11B6 captured on CM5 Chip via Protein A can bind Rhesus macaque Kallikrein 2, His Tag with an affinity constant of 12.48 nM as determined in SPR assay (Biacore T200).
 
Pasritamig captured on CM5 Chip via Protein A can bind Rhesus macaque Kallikrein 2, His Tag with an affinity constant of 34.85 nM as determined in SPR assay (Biacore T200).
背景(Background)

Kallikrein-related peptidase 2 (KLK2) is a serine protease exhibiting antiangiogenic properties through proteolytic activity. KLK2 is overexpressed in prostate cancer and plays a pivotal role in cancer progression, establishing it as a potential therapeutic target.

分子别名(Synonyms)

KLK2; Kallikrein 2; hGK-1; hK2; KLK2A2;hGK1

文献(References)

Jairajpuri DS, Hussain A, Alajmi MF, Mohammad T, Shamsi A, Hassan MI. Structure-based identification of bioactive phytochemicals targeting kallikrein-related peptidase 2 for prostate cancer therapy. Front Chem. 2025 Mar 26;13:1553987. doi: 10.3389/fchem.2025.1553987. PMID: 40207180; PMCID: PMC11979280.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.