Human IL-15 Protein

产品信息(Product Info)

Recombinant Human IL-15 Protein is expressed from E.coli without tag.
It contains Asn49-Ser162 [Accession | P40933].

分子量大小(Molecular Weight)

The protein has a predicted MW of 12.8 kDa same as Tris-Bis PAGE result.


> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC


Less than 0.05 EU per μg by the LAL method.


Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NACI (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.


Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.


-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human IL-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.


The purity of Human IL-15 is greater than 95% as determined by SEC-HPLC.


Immobilized Human IL-15, No Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA.

SPR Data

Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9 nM as determined in SPR assay (Biacore T200).

Cell Based Assay

Measured in a cell proliferation assay using CTLL-2 cells. The ED50 for this effect is 0.1 - 0.3 ng/mL. The specific activity of recombinant human IL-15 is > 1 × 10^7 units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554) (QC Test).


Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.


L15; IL-15; IL-15MGC9721; Interleukin-15


(1) Rautela J , Huntington N D. IL-15 signaling in NK cell cancer immunotherapy [J]. Current Opinion in Immunology, 2017, 44:1-6.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.