IL2-ME422B | 价格

IL2-ME422B-100μg / 询价

IL2-ME422B-500μg / 询价

IL2-ME422B-500μgx2 / 询价

Biotinylated Mouse IL-22 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Mouse IL-22 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.
It contains Leu34-Val179 [Accession | Q9JJY9].

分子量大小(Molecular Weight)

The protein has a predicted MW of 19.7 kDa same as Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 90% as determined by HPLC

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Biotinylated Mouse IL-22 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Biotinylated Mouse IL-22 is greater than 90% as determined by SEC-HPLC.
ELISA Data
Immobilized Biotinylated Mouse IL-22, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test).
SPR Data
Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 μM as determined in SPR assay (Biacore T200).
背景(Background)

The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.

分子别名(Synonyms)

Interleukin-22; IL-22; Cytokine Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; ZCYTO18;IL22; TIFIL23;IL 22; Interleukin 22

文献(References)

(1) Keir M, Yi Y, Lu T, Ghilardi N. The role of IL-22 in intestinal health and disease. J Exp Med. 2020 Feb 13;217(3):e20192195. doi: 10.1084/jem.20192195. PMID: 32997932; PMCID: PMC7062536.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.