GDF-HM128-100μg / 询价
GDF-HM128-500μg / 询价
GDF-HM128-500μgx2 / 询价
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Human Latent GDF-8 Protein
Recombinant Human Latent GDF-8 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Asn24-Ser375 [Accession | O14793].
The protein has a predicted MW of 41.19 kDa. Due to glycosylation, the protein migrates to 14 kDa and 32-38 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 90% as determined by HPLC
Less than 0.1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Myostatin; GDF8; Latent GDF8; Mstn;Growth differentiation factor-8; Growth differentiation factor 8
Walker RG, McCoy JC, Czepnik M, Mills MJ, Hagg A, Walton KL, Cotton TR, Hyvönen M, Lee RT, Gregorevic P, Harrison CA, Thompson TB. Molecular characterization of latent GDF8 reveals mechanisms of activation. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):E866-E875. doi: 10.1073/pnas.1714622115. Epub 2018 Jan 18. PMID: 29348202; PMCID: PMC5798348.
