货号价格

FGF-HM621B-100μg / 询价

FGF-HM621B-500μg / 询价

FGF-HM621B-500μgx2 / 询价

Biotinylated Human FGF21 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human FGF21 Protein is expressed from HEK293 with mFc (IgG1) tag and Avi tag at the N-Terminus. It contains His29-Ser209.[Accession | Q9NSA1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human FGF21 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated Human FGF21, mFc Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.71μg/ml determined by ELISA.

背景(Background)

Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.

分子别名(Synonyms)

UNQ3115; PRO10196; FGF-21; FGF21

文献(References)

(1) Lewis JE, Ebling FJP, Samms RJ, Tsintzas K. Going Back to the Biology of FGF21: New Insights. Trends Endocrinol Metab. 2019;30(8):491-504. doi:10.1016/j.tem.2019.05.007

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.