货号价格

FCR-HM43AB-100μg / 询价

FCR-HM43AB-500μg / 询价

FCR-HM43AB-500μgx2 / 询价

Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly17-Gln208(V176).[Accession | AAH17865]

分子量大小(Molecular Weight)

The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human Fc gamma RIIIA (V176) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human Fc gamma RIIIA (V176) is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 28.2ng/ml determined by ELISA (QC Test).

SPR Data

Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIIA (V176) , His-Avi Tag with an affinity constant of 0.539 μM as determined in SPR assay (Biacore T200).

背景(Background)

The Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

分子别名(Synonyms)

Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20

文献(References)

(1)Koene H R , Kleijer M , Algra J , et al. Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype[J]. Blood, 1997, 90(3):1109-1114.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.