EDA-HM10VB | 价格

EDA-HM10VB-100μg / 询价

EDA-HM10VB-100μgx5 / 询价

EDA-HM10VB-100μgx10 / 询价

Biotinylated Human EDNRA Nanodisc

产品信息(Product Info)
Schematic diagram of Nanodisc preparation

Our Styrene-Maleic Anhydride(SMA) nanodisc platform delivers multi-pass transmembrane proteins exactly as they exist in the HEK293 cell membrane following recombinant expression— preserving their endogenous lipid environment and correct conformational states. Isolated directly from HEK293 cells via a gentle, detergent-free process, the multi-pass membrane proteins are subsequently encapsulated by SMA to ensure their stability. These uniform soluble nanoparticles eliminate extraction interferences while maintaining biological activity. Ensuring consistent performance from lot to lot, our nanodiscs provide the reliability and biological relevance essential for antibody screening and functional characterization in therapeutic antibody drug discovery and development, as assessed by SPR, BLI, ELISA, and other standard platforms.

Features

❖  Correct structure & conformation
❖  Functional relevance ensured
❖  His-tag & biotinylation tag available

Applications

❖  Antibody screening
❖  Functional characterization(Molecular Level)
❖  Assay: SPR, BLI, ELISA

表达区间及表达系统(Source)

Recombinant Biotinylated Human EDNRA Nanodisc is expressed from HEK293 with His tag at the C-terminus.
It contains Met1-Asn427 [Accession | P25101-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 61.70 kDa.

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.

存储(Storage)

Valid for 6 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
ELISA Data
Immobilized Biotinylated Human EDNRA Nanodisc, His Tag 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-EDNRA Antibody, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA. (QC Test)
背景(Background)

The endothelin-A receptor (Ednra) is involved in several physiological, pathological, and developmental pathways. Known for its function in vasoconstriction after being activated by endothelin-1, Ednra also controls cephalic neural crest cell development and appears to play a role in several pathologies, including cancer and periodontitis.

分子别名(Synonyms)

EDNRA; ET-A; ETA-R; hET-AR; ETA; ETRA

文献(References)

Zhang Y, Knutsen GR, Brown MD, Ruest LB. Control of endothelin-a receptor expression by progesterone is enhanced by synergy with Gata2. Mol Endocrinol. 2013 Jun;27(6):892-908. doi: 10.1210/me.2012-1334. Epub 2013 Apr 16. PMID: 23592430; PMCID: PMC3656236.