货号价格

CD3-HM557B-100μg / 询价

CD3-HM557B-500μg / 询价

CD3-HM557B-500μgx2 / 询价

Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G).[Accession | P07766(CD3E)&P09693(CD3G)]

分子量大小(Molecular Weight)

The protein has a predicted MW of 40 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated CD3E&CD3G on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA.

背景(Background)

T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E&CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

分子别名(Synonyms)

CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G

文献(References)

(1)Penaranda C , Tang Q , Bluestone J A. Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.