货号价格

BMP-HE002-100μg / 询价

BMP-HE002-500μg / 询价

BMP-HE002-500μgx2 / 询价

Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein is expressed from E.coli without tag.
It contains Gln283-Arg396 [Accession | P12643].

分子量大小(Molecular Weight)

The protein has a predicted MW of 12.90 kDa same as Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

内毒素(Endotoxin)

Less than 0.2EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 100mM Acetic Acid (pH 2.8). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 100mM Acetic Acid (pH 2.8).

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human/Rhesus macaque/Mouse/Rat/Canine BMP2 on Tris-Bis PAGE under Non reducing (N) condition and reduced condition. The purity is greater than 95%.

Cell Based Assay

Measured by its ability to induce Alkaline Phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 40-200 ng/mL (QC Test).

背景(Background)

Bone morphogenetic protein 2 (BMP2), a member of the transforming growth factor-β (TGF-β) super-family, is one of the main chondrogenic growth factors involved in cartilage regeneration. BMP2 is known to induce chondrogenic differentiation in various types of stem cells in vitro. However, BMP2 also induces osteogenic differentiation and endochondral ossification in mesenchymal stem cells (MSCs).

分子别名(Synonyms)

BMP-2; BMP-2A; BMP2A; BDA2; SSFSC;

文献(References)

Zhou N, Li Q, Lin X, Hu N, Liao JY, Lin LB, Zhao C, Hu ZM, Liang X, Xu W, Chen H, Huang W. BMP2 induces chondrogenic differentiation, osteogenic differentiation and endochondral ossification in stem cells. Cell Tissue Res. 2016 Oct;366(1):101-11. doi: 10.1007/s00441-016-2403-0. Epub 2016 Apr 15. PMID: 27083447.