ACV-HM00B | 价格

ACV-HM00B-100μg / 询价

ACV-HM00B-500μg / 询价

ACV-HM00B-500μgx2 / 询价

Human Activin B Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Activin B Protein is expressed from HEK293 without tag.
It contains Gly293-Ala407 [Accession | P09529].

分子量大小(Molecular Weight)

The protein has a predicted MW of 12.81 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 24-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.22 μm filtered solution in 4mM HCL.

存储(Storage)

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Human Activin B Protein on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
ELISA Data
Immobilized Human Activin B, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag (Cat. ARB-HM22B) with the EC50 of 10.8ng/ml determined by ELISA. (QC Test)
 
Immobilized Human Activin B, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) with the EC50 of 5.4ng/ml determined by ELISA.
BLI Data
Loaded Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) on ProA-Biosensor can bind Human Activin B, No Tag with an affinity constant of 0.25 nM as determined in BLI assay (Gator® Prime).
 
Loaded Human/Cynomolgus Activin RIIB, hFc Tag (Cat. ARB-HM22B) on ProA-Biosensor can bind Human Activin B, No Tag with an affinity constant of 0.19 nM as determined in BLI assay (Gator® Prime).
背景(Background)

The gene inhibin subunit beta B (INHBB), also named Activin B encodes the inhibin βB subunit, which is involved in forming protein members of the transforming growth factor-β (TGF-β) superfamily. The TGF-β superfamily is extensively involved in cell proliferation, differentiation, adhesion, movement, metabolism, communication, and death.

分子别名(Synonyms)

INHBB; Activin B; Inhibin beta B chain;

文献(References)

Liu Y, Zhou Q, Zou G, Zhang W. Inhibin subunit beta B (INHBB): an emerging role in tumor progression. J Physiol Biochem. 2024 Nov;80(4):775-793. doi: 10.1007/s13105-024-01041-y. Epub 2024 Aug 26. PMID: 39183219.