ACV-HM00B | 价格

ACV-HM00B-100μg / 询价

ACV-HM00B-500μg / 询价

ACV-HM00B-500μgx2 / 询价

Human Activin B Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Activin B Protein is expressed from HEK293 without tag.
It contains Gly293-Ala407 [Accession | P09529].

分子量大小(Molecular Weight)

The protein has a predicted MW of 12.81 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 24-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Human Activin B Protein on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Cell Based Assay
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 10 ng/mL. (QC Test)
ELISA Data
Immobilized Human Activin B, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag (Cat. ARB-HM22B) with the EC50 of 10.8ng/ml determined by ELISA. (QC Test)
 
Immobilized Human Activin B, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) with the EC50 of 5.4ng/ml determined by ELISA.
BLI Data
Loaded Human/Cynomolgus Activin RIIA, hFc Tag (Cat. ARA-HM22A) on ProA-Biosensor can bind Human Activin B, No Tag with an affinity constant of 0.25 nM as determined in BLI assay.
 
Loaded Human/Cynomolgus Activin RIIB, hFc Tag (Cat. ARB-HM22B) on ProA-Biosensor can bind Human Activin B, No Tag with an affinity constant of 0.19 nM as determined in BLI assay.
背景(Background)

The gene inhibin subunit beta B (INHBB), also named Activin B encodes the inhibin βB subunit, which is involved in forming protein members of the transforming growth factor-β (TGF-β) superfamily. The TGF-β superfamily is extensively involved in cell proliferation, differentiation, adhesion, movement, metabolism, communication, and death.

分子别名(Synonyms)

INHBB; Activin B; Inhibin beta B chain;

文献(References)

Liu Y, Zhou Q, Zou G, Zhang W. Inhibin subunit beta B (INHBB): an emerging role in tumor progression. J Physiol Biochem. 2024 Nov;80(4):775-793. doi: 10.1007/s13105-024-01041-y. Epub 2024 Aug 26. PMID: 39183219.