货号价格

SIG-HM122-100μg / 询价

SIG-HM122-500μg / 询价

SIG-HM122-500μgx2 / 询价

Human Siglec-2/CD22 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Siglec-2/CD22 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp20-Arg687.[Accession | P20273-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 76.2 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Siglec-2/CD22 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Siglec-2/CD22 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Siglec-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 19.1ng/ml determined by ELISA.

背景(Background)

CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice.

分子别名(Synonyms)

CD22 molecule; CD22; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814

文献(References)

(1)Kato J , Robert T. O'Donnell, Abuhay M , et al. Efficacy and toxicity of a CD22-targeted antibody-saporin conjugate in a xenograft model of non-Hodgkin's lymphoma[J]. OncoImmunology, 2012, 1(9):7.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.