货号价格

PVR-HM301-100μg / 询价

PVR-HM301-500μg / 询价

PVR-HM301-500μgx2 / 询价

Human PVRIG Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human PVRIG Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.
It contains Thr41-Asp171 [Accession | Q6DKI7-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human PVRIG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human PVRIG is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Nectin-2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.17μg/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human PVRIG, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.

SPR Data

Human PVRIG, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 μM as determined in SPR assay (Biacore T200).

背景(Background)

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.

分子别名(Synonyms)

C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1

文献(References)

(1)PVRIG and PVRL2 Are Induced in Cancer and Inhibit CD8 T-cell Function. 2019.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.