货号价格

MSL-MM180-100μg / 询价

MSL-MM180-500μg / 询价

MSL-MM180-500μgx2 / 询价

Mouse MSLN/Mesothelin Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse MSLN/Mesothelin Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Asp298-Ser600 [Accession | Q61468-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Mouse MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse MSLN is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Mouse MSLN, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 21.7ng/ml determined by ELISA.

背景(Background)

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF).

分子别名(Synonyms)

MSLN; CAK1 antigen; CAK1; Mesothelin; MPF; MPFSMRP; SMR;

文献(References)

(1)Adusumilli P S , Cherkassky L , Villena-Vargas J , et al. Regional delivery of mesothelin-targeted CAR T cell therapy generates potent and long-lasting CD4-dependent tumor immunity[J]. Science Translational Medicine, 2014, 6(261):261ra151-261ra151.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.