MSL-MM180-100μg / 询价
MSL-MM180-500μg / 询价
MSL-MM180-500μgx2 / 询价
Mouse MSLN/Mesothelin Protein
Recombinant Mouse MSLN/Mesothelin Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp298-Ser600.[Accession | Q61468-1]
The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse MSLN is greater than 95% as determined by SEC-HPLC.
Immobilized Mouse MSLN, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 21.7ng/ml determined by ELISA.
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF).
MSLN; CAK1 antigen; CAK1; Mesothelin; MPF; MPFSMRP; SMR;
(1)Adusumilli P S , Cherkassky L , Villena-Vargas J , et al. Regional delivery of mesothelin-targeted CAR T cell therapy generates potent and long-lasting CD4-dependent tumor immunity[J]. Science Translational Medicine, 2014, 6(261):261ra151-261ra151.
