货号价格

MSL-HM480F-100μg / 询价

MSL-HM480F-500μg / 询价

MSL-HM480F-1mg / 询价

FITC-Labeled Human MSLN/Mesothelin Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant FITC-Labeled Human MSLN/Mesothelin Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu296-Gly580.[Accession | Q13421-2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

制剂(Formulation)

Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.

存储(Storage)

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

FITC-Labeled Human MSLN/Mesothelin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

FACS Data

FACS Analysis of Anti-MSLN CAR Expression. 293T cells were transfected with anti-MSLN-scFv and His tag. Cells were stained with 5μg/ml FITC-Labeled Human MSLN (296-580) , His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.

背景(Background)

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

分子别名(Synonyms)

MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR

文献(References)

(1)Adusumilli P S , Cherkassky L , Villena-Vargas J , et al. Regional delivery of mesothelin-targeted CAR T cell therapy generates potent and long-lasting CD4-dependent tumor immunity[J]. Science Translational Medicine, 2014, 6(261):261ra151-261ra151.

(2)Morello A , Sadelain M , Adusumilli P S. Mesothelin-Targeted CARs: Driving T cells to Solid Tumors[J]. Cancer Discovery, 2015, 6(2).

恺佧生物科技(上海)有限公司
电话:400-614-0008
地址:上海市浦江高科技园新骏环路188号8A区四楼
在线留言