货号价格

MSL-HM480-100μg / 询价

MSL-HM480-500μg / 询价

MSL-HM480-1mg / 询价

Human MSLN/Mesothelin Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human MSLN/Mesothelin Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu296-Gly580.[Accession | Q13421-2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human MSLN/Mesothelin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human MSLN/Mesothelin was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human MSLN, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-MSLN Ab., hFc Tag with the EC50 of 15.84ng/ml determined by ELISA.

背景(Background)

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

分子别名(Synonyms)

MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR

文献(References)

(1)Adusumilli P S , Cherkassky L , Villena-Vargas J , et al. Regional delivery of mesothelin-targeted CAR T cell therapy generates potent and long-lasting CD4-dependent tumor immunity[J]. Science Translational Medicine, 2014, 6(261):261ra151-261ra151.

(2)Morello A , Sadelain M , Adusumilli P S. Mesothelin-Targeted CARs: Driving T cells to Solid Tumors[J]. Cancer Discovery, 2015, 6(2).

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