货号价格

MSL-CM280-100μg / 询价

MSL-CM280-500μg / 询价

MSL-CM280-500μgx2 / 询价

Cynomolgus MSLN/Mesothelin Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus MSLN/Mesothelin Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Asp296-Gly580.[Accession | A0A2K5TW94-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Cynomolgus MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus MSLN is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Cynomolgus MSLN, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-MSLN Antibody, hFc Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test).

 

Immobilized Human CA125, His Tag at 5ug/ml (100μl/Well). Dose response curve for Cynomolgus MSLN, hFc Tag with the EC50 of 4.8μg/ml determined by ELISA.

背景(Background)

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

分子别名(Synonyms)

MSLN; CAK1 antigen; CAK1; Mesothelin; MPF; MPFSMRP; SMR;

文献(References)

(1)Adusumilli P S , Cherkassky L , Villena-Vargas J , et al. Regional delivery of mesothelin-targeted CAR T cell therapy generates potent and long-lasting CD4-dependent tumor immunity[J]. Science Translational Medicine, 2014, 6(261):261ra151-261ra151.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.