货号价格

MHC-HM418T-100μg / 询价

MHC-HM418T-500μg / 询价

MHC-HM418T-1mg / 询价

Human KRAS G12V (HLA-A*03:01) Complex Tetramer Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human KRAS G12V (HLA-A*03:01) Complex Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide.[Accession | NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVGAVGVGK]

分子量大小(Molecular Weight)

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per ug by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human KRAS G12V (HLA-A*03:01) Complex Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human KRAS G12V (HLA-A*03:01) Complex Tetramer is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human KRAS G12V (HLA-A*03:01) Complex Tetramer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-KRAS G12V (HLA-A*03:01) Antibody, hFc Tag with the EC50 of 0.58μg/ml determined by ELISA.

SPR Data

Anti-KRAS G12V (HLA-A*03:01) Antibody captured on CM5 Chip via Protein A can bind Human KRAS G12V (HLA-A*03:01) Tetramer, His Tag with an affinity constant of 42.3 nM as determined in SPR assay (Biacore T200).

背景(Background)

Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.

分子别名(Synonyms)

MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD

文献(References)

(1) Wu HZ, Xiao JQ, Xiao SS, Cheng Y. KRAS: A Promising Therapeutic Target for Cancer Treatment. Curr Top Med Chem. 2019;19(23):2081-2097. doi: 10.2174/1568026619666190905164144. PMID: 31486755.

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