货号价格

MHC-HM405-100μg / 询价

MHC-HM405-500μg / 询价

MHC-HM405-1mg / 询价

Human NY-ESO-1 (HLA-A*02:01) Complex Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human NY-ESO-1 (HLA-A*02:01) Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQC peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC]

分子量大小(Molecular Weight)

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.

存储(Storage)

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human NY-ESO-1 (HLA-A*02:01) Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human NY-ESO-1 (HLA-A*02:01) Complex was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human NY-ESO-1 (HLA-A*02:01) Complex, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NY-ESO-1 (HLA-A*0201) Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA.

SPR Data

Anti-hFc IgG capture Anti-NY-ESO-1 (HLA-A*02:01) Antibody, Human NY-ESO-1 (HLA-A*02:01) Complex as analyte; The affinity constant of 13.7nM as determined in SPR assay (Biacore T200).

背景(Background)

NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy.

分子别名(Synonyms)

MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1

文献(References)

(1)Thomas R, Al-Khadairi G, Roelands J, Hendrickx W, Dermime S, Bedognetti D, Decock J. NY-ESO-1 Based Immunotherapy of Cancer: Current Perspectives. Front Immunol. 2018 May 1;9:947. doi: 10.3389/fimmu.2018.00947. PMID: 29770138; PMCID: PMC5941317.

恺佧生物科技(上海)有限公司
电话:400-614-0008
地址:上海市浦江高科技园新骏环路188号8A区四楼
在线留言