货号价格

MHC-HM401-100μg / 询价

MHC-HM401-500μg / 询价

MHC-HM401-1mg / 询价

Human MAGE-A4 (HLA-A*02:01) Complex Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human MAGE-A4 (HLA-A*02:01) Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and GVYDGREHTV peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV]

分子量大小(Molecular Weight)

The protein has a predicted MW of 50.5 kDa. Due to glycosylation,the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human MAGE-A4 (HLA-A*02:01) Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human MAGE-A4 (HLA-A*02:01) Complex is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human MAGE-A4 (HLA-A*02:01) Complex, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA.

背景(Background)

Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and GVYDGREHTV peptide of  the MAGE-A4.

分子别名(Synonyms)

HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4

文献(References)

(1)Kageyama S , Ikeda H , Miyahara Y , et al. Adoptive Transfer of MAGE-A4 T-cell Receptor Gene-Transduced Lymphocytes in Patients with Recurrent Esophageal Cancer[J]. Clinical Cancer Research, 2015, 21(10):2268.

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