货号价格

HLG-HE41F-100μg / 询价

HLG-HE41F-500μg / 询价

HLG-HE41F-500μgx2 / 询价

Human HLA-G Free Heavy Chain Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human HLA-G Free Heavy Chain Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(C66S).[Accession | P17693-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.5 kDa. The protein migrates to 36-40 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

制剂(Formulation)

Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl, 20% Glycerol (pH 8.0).

存储(Storage)

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human HLA-G Free heavy chain on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human HLA-G Free Heavy Chain, His Tag at 2μg/ml (100μl/Well) on the. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 29.0ng/ml determined by ELISA.

背景(Background)

HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.

分子别名(Synonyms)

HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G

文献(References)

(1) Krijgsman D, Roelands J, Hendrickx W, Bedognetti D, Kuppen PJK. HLA-G: A New Immune Checkpoint in Cancer? Int J Mol Sci. 2020 Jun 25;21(12):4528. doi: 10.3390/ijms21124528. PMID: 32630545; PMCID: PMC7350262.