货号价格

HER-HM402-100μg / 询价

HER-HM402-500μg / 询价

HER-HM402-1mg / 询价

Human Her2/ErbB2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Her2/ErbB2 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Thr23-Thr652.[Accession | P04626-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 72.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Her2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Her2 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Her2, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA.

背景(Background)

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane.

分子别名(Synonyms)

CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; NGL; TKR1; ERBB2; NEU; ENV; ENVW; ERVWE1; HERV7Q; HERVW

文献(References)

(1)Tagliabue E , Balsari A , Campiglio M , et al. HER2 as a target for breast cancer therapy[J]. EXPERT OPINION ON BIOLOGICAL THERAPY, 2010, 10(5):711-724.

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