货号价格

GPC-HM131-100μg / 询价

GPC-HM131-500μg / 询价

GPC-HM131-1mg / 询价

Human GPC3/Glypican 3 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human GPC3/Glypican 3 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln25-His559.[Accession | P51654-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 70-115 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

SEC-HPLC

The purity of Human GPC3 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human GPC3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human GPC3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 11.7ng/ml / 11.3ng/ml / 14.3ng/ml determined by ELISA.

背景(Background)

Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

分子别名(Synonyms)

GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1

文献(References)

(1)Ho M , Kim H. Glypican-3: A new target for cancer immunotherapy[J]. European Journal of Cancer, 2011, 47(3):0-338.

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