货号价格

DAM-HM101-100μg / 询价

DAM-HM101-500μg / 询价

DAM-HM101-500μgx2 / 询价

Human DNAM-1/CD226 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human DNAM-1/CD226 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu19-Asn247.[Accession | Q15762]

分子量大小(Molecular Weight)

The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human DNAM-1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human DNAM-1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 0.24μg/ml determined by ELISA (QC Test).

SPR Data

Human CD155, hFc Tag captured on Protein A chip, can bind Human DNAM-1, His Tag with an affinity constant of 0.22μM as determined in a SPR assay (Biacore T200).

背景(Background)

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

分子别名(Synonyms)

CD226 molecule; CD226; DNAM1; PTA1; TLiSA1

文献(References)

(1)Yu X , Harden K , C Gonzalez L , et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.