货号价格

CD7-HM401-100μg / 询价

CD7-HM401-500μg / 询价

CD7-HM401-500μgx2 / 询价

Human CD7 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human CD7 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala26-Pro180.[Accession | P09564]

分子量大小(Molecular Weight)

The protein has a predicted MW of 19.3 kDa. Due to glycosylation, the protein migrates to 32-45 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human CD7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD7 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CD7, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 3.2ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human CD7, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.

背景(Background)

CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily.CD7 is expressed on T cells, NK cells , myeloid progenitor cells, and CD19 B progenitor cells. Among CD8 T cells, the CD7-bright population preferentially contains naïve and memory cells, while more weak expressors are primarily effector cells.

分子别名(Synonyms)

CD7 molecule; CD7; LEU-9; Tp40; TP41; GP40

文献(References)

(1)Gomes-Silva D , Srinivasan M , Sharma S , et al. CD7-edited T cells expressing a CD7-specific CAR for the therapy of T-cell malignancies[J]. Blood, 2017:blood-2017-01-761320.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.