恺佧生物科技（上海）有限公司

表达区间及表达系统（Source）	Recombinant Human Siglec-3/CD33 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Asp18-His259.[Accession \| P20138-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
存储（Storage）	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

表达区间及表达系统（Source）

Recombinant Human Siglec-3/CD33 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Asp18-His259.[Accession | P20138-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储（Storage）

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.