Human VEGF165 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human VEGF165 Protein is expressed from HEK293 without tag.
It contains Ala27-Arg191 [Accession | P15692-4].

分子量大小(Molecular Weight)

The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.05EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human VEGF165 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 46.4ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA.

Cell Based Assay

Human VEGF165 stimulates proliferation of human umbilical vein endothelial cells (HUVEC) with the ED50 for this effect is 2.57ng/mL (QC Test).

背景(Background)

Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.

分子别名(Synonyms)

VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165

文献(References)

(1) Patel KA, Patel BM, Thobias AR, Gokani RA, Chhikara AB, Desai AD, Patel PS. Overexpression of VEGF165 is associated with poor prognosis of cervical cancer. J Obstet Gynaecol Res. 2020 Nov;46(11):2397-2406. doi: 10.1111/jog.14483. Epub 2020 Sep 27. PMID: 32985053.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.