货号价格

MHC-HM413T-100μg / 询价

MHC-HM413T-500μg / 询价

MHC-HM413T-1mg / 询价

Human&Mouse Chimeric LMP2 (HLA-A*02:01) Tetramer Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human&Mouse Chimeric LMP2 (HLA-A*02:01) Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119(B2M) and CLGGLLTMV peptide.[Accession | A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse HLA-A*02:01 α3)&P61769(B2M)&CLGGLLTMV]

分子量大小(Molecular Weight)

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.

存储(Storage)

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human&Mouse Chimeric LMP2 (HLA-A*02:01) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human&Mouse Chimeric LMP2 (HLA-A*02:01) Tetramer was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human&Mouse Chimeric LMP2 (HLA-A*02:01) Tetramer, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LMP2 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA.

背景(Background)

The immunoproteasome, having been linked to neurodegenerative diseases and hematological cancers, has been shown to play an important role in MHC class I antigen presentation. The development of molecular probes that selectively inhibit the major catalytic subunit, LMP2, of the immunoproteasome,LMP2-rich cancer cells compared to LMP2-deficient cancer cells are more sensitive to growth inhibition by the LMP2-specific inhibitor, implicating an important role of LMP2 in regulating cell growth of malignant tumors that highly express LMP2.

分子别名(Synonyms)

MHC; RMF; LMP2; LMP-2; Macropain chain 7; Proteasome chain 7; PSMB9; RING12

文献(References)

(1) Ho YK, Bargagna-Mohan P, Wehenkel M, Mohan R, Kim KB. LMP2-specific inhibitors: chemical genetic tools for proteasome biology. Chem Biol. 2007 Apr;14(4):419-30. doi: 10.1016/j.chembiol.2007.03.008. PMID: 17462577; PMCID: PMC5541682.

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