ITG-HM447-100μg / 询价
ITG-HM447-500μg / 询价
ITG-HM447-500μgx2 / 询价
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Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein
Recombinant Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr34-Thr977(ITGA4) acidic tail and Glu20-His723(ITGB7) basic tail [Accession | P13612-1(ITGA4)&P26010-1(ITGB7)].
The protein has a predicted MW of 112.5 kDa (ITGA4) & 81.3 kDa (ITGB7). Due to glycosylation,the protein migrates to 70 kDa, 80 kDa, 100-120 kDa and 130-150 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 90% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human ITGA4&ITGB7 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human ITGA4&ITGB7 is greater than 90% as determined by SEC-HPLC.
Immobilized Human ITGA4&ITGB7, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-ITGA4 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
Immobilized Human ITGA4&ITGB7, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human MADCAM1, hFc Tag with the EC50 of 5.5μg/ml determined by ELISA.
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1.
Integrin alpha 4 beta 7; beta7; alpha 4; LPAM; ITGA4&ITGB7;Integrin alpha 4 β 7; β7
(1)Petrovic A , Alpdogan O , Willis L M , et al. LPAM (alpha 4 beta 7 integrin) is an important homing integrin on alloreactive T cells in the development of intestinal graft-versus-host disease.[J]. Blood, 2004, 103(4):1542.
