ILT-HM107-100μg / 询价
ILT-HM107-500μg / 询价
ILT-HM107-500μgx2 / 询价
Human IL-7 R alpha&TSLPR Protein
Recombinant Human IL-7 R alpha&TSLPR Protein is expressed from HEK293 with His tag at the C-terminus of IL-7 R alpha.It contains Glu21-Asp239 (IL-7 R alpha) and Gln23-Lys231 (TSLPR) [Accession | P16871-1(IL-7 R alpha)&Q9HC73-1(TSLPR)].
The protein has a predicted MW of 31.18 kDa (IL-7 R alpha) and 29.03 kDa (TSLPR). Due to glycosylation, the protein migrates to 58-68 kDa (IL-7 R alpha) and 50-58 kDa (TSLPR) based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-7 R alpha&TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-7 R alpha&TSLPR is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-7R alpha&TSLPR, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TSLP, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Human IL-7R alpha&TSLPR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-7, No Tag with an affinity constant of 15.54 nM as determined in SPR assay (Biacore T200).
Loaded Human TSLP, Fc Tag on ProA-Biosensor can bind IL-7 R alpha&TSLP R, His Tag with an affinity constant of 0.37 nM as determined in BLI assay (Gator® Prime).
IL-7R alpha & TSLPR is an heterodimeric receptor, with TSLP as the ligand, which initiates downstream signaling pathways upon binding to TSLP and subsequently participates in thymus development and immune cell generation. Among them, IL-7R alpha is a component of both the IL-7 and TSLP receptors.
IL7Ra; CD127; TSLP R; CRLF2; IL-XR; ILXR; p2RY8; TSLPR; CRL2; CRLF2Y
(1) Durum SK. IL-7 and TSLP receptors: twisted sisters. Blood. 2014 Jul 3;124(1):4-5. doi: 10.1182/blood-2014-05-574327.
(2) Pandey A, Ozaki K, Baumann H, Levin SD, Puel A, Farr AG, Ziegler SF, Leonard WJ, Lodish HF. Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin. Nat Immunol. 2000 Jul;1(1):59-64. doi: 10.1038/76923.
(3) Sheikh A, Abraham N. Interleukin-7 Receptor Alpha in Innate Lymphoid Cells: More Than a Marker. Front Immunol. 2019 Dec 11;10:2897. doi: 10.3389/fimmu.2019.02897.
