ILR-DM123 | 价格

ILR-DM123-100μg / 询价

ILR-DM123-500μg / 询价

ILR-DM123-500μgx2 / 询价

Canine IL-23R Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Canine IL-23R Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Gly24-Asp354 [Accession | A0A8I3PXL0].

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.31 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Canine IL-23R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Canine IL-23R is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Canine IL-23R, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 2.7μg/ml determined by ELISA.

背景(Background)

Interleukin-23 (IL-23), a member of the IL-12 family of cytokines, is a heterodimeric cytokine. It is composed of subunits p40 (shared with IL-12) and p19 (an IL-12 p35-related subunit) and is secreted by several types of immune cells, such as natural killer cells and dendritic cells. The IL-23 receptor is composed of the subunit IL-12Rβ1 and the IL-23-specific subunit IL-23R. The binding of IL-23 to its specific cell surface receptor regulates a number of functions, including proliferation and differentiation of cells and secretion of cell factors.

分子别名(Synonyms)

IBD17; IL-23 R; IL-23 receptor

文献(References)

Li Y, Wang H, Lu H, Hua S. Regulation of Memory T Cells by Interleukin-23. Int Arch Allergy Immunol. 2016;169(3):157-62. doi: 10.1159/000445834. Epub 2016 Apr 22. PMID: 27100864.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.