货号价格

IL5-MM2RA-100μg / 询价

IL5-MM2RA-500μg / 询价

IL5-MM2RA-500μgx2 / 询价

Mouse IL-15RA/IL-15 R alpha/CD215 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse IL-15RA/IL-15 R alpha/CD215 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gly33-Lys205.[Accession | Q60819-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 45.1 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Mouse IL-15RA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse IL-15RA is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human IL-15, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse IL-15RA, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA.

背景(Background)

Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.

分子别名(Synonyms)

IL-15 R alpha; CD215; IL-15RA; MGC104179

文献(References)

(1)Nadeau L, Aguer C. Interleukin-15 as a myokine: mechanistic insight into its effect on skeletal muscle metabolism. Appl Physiol Nutr Metab. 2019 Mar;44(3):229-238. doi: 10.1139/apnm-2018-0022. Epub 2018 Sep 6. PMID: 30189147.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.