货号价格

IL2-HE021-100μg / 询价

IL2-HE021-500μg / 询价

IL2-HE021-500μgx2 / 询价

Human IL-21 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-21 Protein is expressed from E.coli without tag.
It contains Gln32-Ser162 [Accession | Q9HBE4-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 15.4 kDa same as Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.5).

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human IL-21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human IL-21, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA.

Cell Based Assay

Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 1-3 ng/mL (QC Test).

背景(Background)

Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.

分子别名(Synonyms)

Interleukin-21; IL-21; Za11; CVID11

文献(References)

(1)Sim GC, Radvanyi L. The IL-2 cytokine family in cancer immunotherapy. Cytokine Growth Factor Rev. 2014 Aug;25(4):377-90. doi: 10.1016/j.cytogfr.2014.07.018. Epub 2014 Aug 1. PMID: 25200249.