IL1-HE013-100μg / 询价
IL1-HE013-500μg / 询价
IL1-HE013-500μgx2 / 询价
Human IL-13 Protein
Recombinant Human IL-13 Protein is expressed from E.coli without tag.It contains Gly21-Asn132 [Accession | AAK53823].
The protein has a predicted MW of 12.34 kDa same as Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 0.1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-13 is greater than 95% as determined by SEC-HPLC.
Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/mL. (QC Test)
Immobilized Human IL-13, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 3.6ng/ml determined by ELISA. (QC Test)
Loaded Human IL-13Ra2, His Tag on Anti-His-Biosensor can bind Human IL-13, No Tag with an affinity constant of 0.63 nM as determined in BLI assay (Gator® Prime).
KACTUS GMP grade cytokines are manufactured and tested in accordance with relevant GMP guidelines and regulatory requirements, and are in compliance with ISO 13485:2016 certified quality system.
● Personnel training programs
● Clean rooms and facility
● Facility maintenance and equipment calibration & verification
● Raw material testing and supplier evaluation and approval
● Animal-free manufacturing
● Rigorous quality control
● Stability testing(freeze-thaw and accelerated stability of reconstituted protein; long-term stability in lyophilized state)
● Comprehensive support files, including COA, COO, MSDS, TSE/BSE statement, etc.
We conduct comprehensive quality control testing to ensure that KACTUS GMP grade cytokines meet strict standards for identification, purity, safety, bioactivity and batch-to-batch consistency.
● Identification by Bis-Tris PAGE
● Purity by SEC-HPLC
● Endotoxin level
● Residual host cell protein
● Residual host cell DNA
● Mycoplasma testing
● Sterility testing
● Bioactivity (Specific activity against standards of WHO if applicable)
● Batch-to-batch consistency
Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
(1)Grünig G, Corry D B , Reibman J , et al. Interleukin 13 and the evolution of asthma therapy[J]. American Journal of Clinical & Experimental Immunology, 2012, 1(1):20.
KACTUS Human IL-13 Protein is for research or ex vivo cell processing use, not for human or animal in vivo application.
