Human HVEM/TNFRSF14 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu39-Val202.[Accession | Q92956-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human HVEM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human BTLA, His Tag at 1μg/ml (100μl/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 42.5ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human LIGHT, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA.

SPR Data

Human HVEM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD160, His Tag with an affinity constant of 1.44 μM as determined in SPR assay (Biacore T200).

背景(Background)

Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily.  Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.

分子别名(Synonyms)

CD270; CD40-like protein;  HVEM; LIGHTR; ATAR; TNFRSF14; TR2

文献(References)

(1)Granger S W, Rickert S. LIGHT-HVEM signaling and the regulation of T cell-mediated immunity[J]. Cytokine Growth Factor Rev, 2003, 14(3):289-296.