Human HVEM/TNFRSF14 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Leu39-Val202 [Accession | Q92956-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human HVEM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human HVEM is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA (QC Test).

 

Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.

ELISA Data

Immobilized Human HVEM, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.

背景(Background)

Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily.  Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.

分子别名(Synonyms)

CD270; CD40-like protein;  HVEM; LIGHTR; ATAR; TNFRSF14; TR2

文献(References)

(1)Granger S W, Rickert S. LIGHT-HVEM signaling and the regulation of T cell-mediated immunity[J]. Cytokine Growth Factor Rev, 2003, 14(3):289-296.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.