货号价格

GPR-CM05P-100μg / 询价

GPR-CM05P-100μg x 5 / 询价

GPR-CM05P-100μg x 10 / 询价

Cynomolgus GPRC5D Protein-VLP

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus GPRC5D Protein-VLP is expressed from HEK293.; It contains Met1-Cys300.[Accession | A0A2K5W6I7]

分子量大小(Molecular Weight)

The target protein has a predicted MW of 35.10 kDa.

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by HPLC

制剂(Formulation)

Supplied as 0.22 μm filtered solution in PBS, 300 mM L-Arginine (pH 7.4). Notice: If you need it for immunization, Do Not use any adjuvant.

存储(Storage)

Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
SEC-HPLC

The purity of Cynomolgus GPRC5D VLP is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Cynomolgus GPRC5D VLP (100 μl/well) at 5 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 6.91 μg/ml determined by ELISA.

背景(Background)

Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.

分子别名(Synonyms)

GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor

文献(References)

(1) Smith EL, Harrington K, Staehr M, Masakayan R, Jones J, Long TJ, Ng KY, Ghoddusi M, Purdon TJ, Wang X, Do T, Pham MT, Brown JM, De Larrea CF, Olson E, Peguero E, Wang P, Liu H, Xu Y, Garrett-Thomson SC, Almo SC, Wendel HG, Riviere I, Liu C, Sather B, Brentjens RJ. GPRC5D is a target for the immunotherapy of multiple myeloma with rationally designed CAR T cells. Sci Transl Med. 2019 Mar 27;11(485):eaau7746. doi: 10.1126/scitranslmed.aau7746. PMID: 30918115; PMCID: PMC7508042.