货号价格

FLT-HE03L-100μg / 询价

FLT-HE03L-500μg / 询价

FLT-HE03L-500μgx2 / 询价

Human FLT3 Ligand Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human FLT3 Ligand Protein is expressed from E.coli without tag.
It contains Thr27-Ala181 [Accession | P49771-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 17.61 kDa same as Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human FLT3 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human FLT3 Ligand, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 21.7ng/ml determined by ELISA.

Cell Based Assay

The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 1.0 ng/ml. (QC Test)

背景(Background)

Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

分子别名(Synonyms)

FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine

文献(References)

(1)Small D. Targeting FLT3 for the Treatment of Leukemia[J]. Seminars in Hematology, 2008, 45(supp-S2).

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.