货号价格

EG8-HM154F-100μg / 询价

EG8-HM154F-500μg / 询价

EG8-HM154F-1mg / 询价

FITC-Labeled Human EGFRVIII Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant FITC-Labeled Human EGFRVIII Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu25-Ser378.[Accession | NP_001333870.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

制剂(Formulation)

Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.

存储(Storage)

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

FITC-Labeled Human EGFRVIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

FACS Data

FACS Analysis of Anti-EGFRVIII CAR Expression. 293T cells were transfected with anti-EGFRVIII-scFv and His tag. Cells were incubated with 5μg/ml FITC-Labeled Human EGFRVIII, His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.

背景(Background)

The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.

分子别名(Synonyms)

ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2

文献(References)

(1)Pedersen M W , Meltorn M , Damstrup L , et al. The type III epidermal growth factor receptor mutation Biological significance and potential target for anti-cancer therapy[J]. Annals of Oncology, 2001, 12(6):745-760.

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