货号价格

EG8-HM154F-100μg / 询价

EG8-HM154F-500μg / 询价

EG8-HM154F-500μgx2 / 询价

FITC-Labeled Human EGFRVIII Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant FITC-Labeled Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu25-Ser378.[Accession | NP_001333870.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.

波长(Wavelength)

Excitation Wavelength: 490 nm

Emission Wavelength: 520 nm

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

制剂(Formulation)

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

存储(Storage)

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

FITC-Labeled Human EGFRVIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

FACS Data

FACS Analysis of Anti-EGFRVIII CAR Expression. 293T cells were transfected with anti-EGFRVIII-scFv and His tag. Cells were incubated with 5μg/ml FITC-Labeled Human EGFRVIII, His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.

背景(Background)

The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.

分子别名(Synonyms)

ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2

文献(References)

(1)Pedersen M W , Meltorn M , Damstrup L , et al. The type III epidermal growth factor receptor mutation Biological significance and potential target for anti-cancer therapy[J]. Annals of Oncology, 2001, 12(6):745-760.