货号价格

BCM-CM217-100μg / 询价

BCM-CM217-500μg / 询价

BCM-CM217-500μgx2 / 询价

Cynomolgus/Rhesus macaque BCMA/TNFRSF17 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus/Rhesus macaque BCMA/TNFRSF17 Protein is expressed from HEK293 with hFc at the C-Terminus. It contains Met1-Ala53.[Accession | A0A2K5UD97 / F7FFA0]

分子量大小(Molecular Weight)

The protein has a predicted MW of 31.5 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Cynomolgus/Rhesus macaque BCMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus/Rhesus macaque BCMA is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Cynomolgus/Rhesus macaque BCMA, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-BCMA Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA.

ELISA Data

Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.

背景(Background)

B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).

分子别名(Synonyms)

CD269; TNFRSF17; BCMA; BCM; TNFRSF13A

文献(References)

(1)Bossen C , Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.