Mouse APRIL/TNFSF13 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse APRIL/TNFSF13 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala96-Leu241.[Accession | Q9D777]

分子量大小(Molecular Weight)

The protein has a predicted MW of 43.20 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Mouse APRIL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse APRIL is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Mouse APRIL, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA.

背景(Background)

The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.

分子别名(Synonyms)

CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2

文献(References)

(1)Bossen C , Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.