Human Activin A Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Activin A Protein is expressed from HEK293 without tag.
It contains Gly311-Ser426 [Accession | P08476].

分子量大小(Molecular Weight)

The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

内毒素(Endotoxin)

Less than 0.1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCI.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Activin A on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

ELISA Data

Immobilized Human Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.

SPR Data

Biotinylated Human Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).

Cell Based Assay

Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test).

背景(Background)

Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.

分子别名(Synonyms)

INHBA; Activin A;

文献(References)

Morianos I, Papadopoulou G, Semitekolou M, Xanthou G. Activin-A in the regulation of immunity in health and disease. J Autoimmun. 2019 Nov;104:102314. doi: 10.1016/j.jaut.2019.102314. Epub 2019 Aug 13. PMID: 31416681.