货号价格

WNT-HM23A-100μg / 询价

WNT-HM23A-500μg / 询价

WNT-HM23A-500μgx2 / 询价

Human Wnt Surrogate-Fc Fusion Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Wnt Surrogate-Fc Fusion Protein is expressed from HEK293 with hFc tag at the C-Terminus

分子量大小(Molecular Weight)

The protein has a predicted MW of 58.50 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Wnt Surrogate-Fc Fusion on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Cell Based Assay

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 5.2 ng/mL.

背景(Background)

Wnt-3a is regarded as an activator of the canonical Wnt signaling pathway. This activator is expressed in the dorsal midline region and is responsible for spinal cord development. In addition, Wnt-3a plays a regulatory role in autophagy, apoptosis, and regeneration of neurons, neurogenic inflammation and axon regeneration.Wnt-3a promotes the beta-catenin/Tcf pathway which is tumor inducing and can cause cancer when expressed in particular cell populations. It is also one of the most commonly used cytokines for organoid construction.

分子别名(Synonyms)

Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a

文献(References)

[1] Gao K, Zhang T, Wang F, Lv C. Therapeutic Potential of Wnt-3a in Neurological Recovery after Spinal Cord Injury. Eur Neurol. 2019;81(3-4):197-204. doi: 10.1159/000502004. Epub 2019 Jul 23. PMID: 31336380.

[2] Pashirzad M, Fiuji H, Khazei M, Moradi-Binabaj M, Ryzhikov M, Shabani M, Avan A, Hassanian SM. Role of Wnt3a in the pathogenesis of cancer, current status and prospective. Mol Biol Rep. 2019 Oct;46(5):5609-5616. doi: 10.1007/s11033-019-04895-4. Epub 2019 Jun 24. PMID: 31236761.