WNT-HM23A-100μg / 询价
WNT-HM23A-500μg / 询价
WNT-HM23A-500μgx2 / 询价
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Human Wnt Surrogate-Fc Fusion Protein
Recombinant Human Wnt Surrogate-Fc Fusion Protein is expressed from HEK293 with hFc tag at the C-Terminus
The protein has a predicted MW of 58.50 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Wnt Surrogate-Fc Fusion on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2-12 ng/mL (QC Test).
KACTUS GMP grade cytokines are manufactured and tested in accordance with relevant GMP guidelines and regulatory requirements, and are in compliance with ISO 13485:2016 certified quality system.
● Personnel training programs
● Clean rooms and facility
● Facility maintenance and equipment calibration & verification
● Raw material testing and supplier evaluation and approval
● Animal-free manufacturing
● Rigorous quality control
● Stability testing(freeze-thaw and accelerated stability of reconstituted protein; long-term stability in lyophilized state)
● Comprehensive support files, including COA, COO, MSDS, TSE/BSE statement, etc.
We conduct comprehensive quality control testing to ensure that KACTUS GMP grade cytokines meet strict standards for identification, purity, safety, bioactivity and batch-to-batch consistency.
● Identification by Bis-Tris PAGE
● Purity by SEC-HPLC
● Endotoxin level
● Residual host cell protein
● Residual host cell DNA
● Mycoplasma testing
● Sterility testing
● Bioactivity (Specific activity against standards of WHO if applicable)
● Batch-to-batch consistency
Wnt-3a is regarded as an activator of the canonical Wnt signaling pathway. This activator is expressed in the dorsal midline region and is responsible for spinal cord development. In addition, Wnt-3a plays a regulatory role in autophagy, apoptosis, and regeneration of neurons, neurogenic inflammation and axon regeneration.Wnt-3a promotes the beta-catenin/Tcf pathway which is tumor inducing and can cause cancer when expressed in particular cell populations. It is also one of the most commonly used cytokines for organoid construction.
Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a
[1] Gao K, Zhang T, Wang F, Lv C. Therapeutic Potential of Wnt-3a in Neurological Recovery after Spinal Cord Injury. Eur Neurol. 2019;81(3-4):197-204. doi: 10.1159/000502004. Epub 2019 Jul 23. PMID: 31336380.
[2] Pashirzad M, Fiuji H, Khazei M, Moradi-Binabaj M, Ryzhikov M, Shabani M, Avan A, Hassanian SM. Role of Wnt3a in the pathogenesis of cancer, current status and prospective. Mol Biol Rep. 2019 Oct;46(5):5609-5616. doi: 10.1007/s11033-019-04895-4. Epub 2019 Jun 24. PMID: 31236761.
KACTUS Human Wnt Surrogate-Fc Fusion Protein is for research or ex vivo cell processing use, not for human or animal in vivo application.
