WNT-HM23A-100μg / 询价

WNT-HM23A-500μg / 询价

WNT-HM23A-500μgx2 / 询价

Human Wnt Surrogate-Fc Fusion Protein

产品信息(Product Info)

Recombinant Human Wnt Surrogate-Fc Fusion Protein is expressed from HEK293 with hFc tag at the C-Terminus

分子量大小(Molecular Weight)

The protein has a predicted MW of 58.50 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.


> 95% as determined by Tris-Bis PAGE


Less than 1EU per μg by the LAL method.


Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.


Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.


-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Wnt Surrogate-Fc Fusion on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Cell Based Assay

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 5.2 ng/mL.


Wnt-3a is regarded as an activator of the canonical Wnt signaling pathway. This activator is expressed in the dorsal midline region and is responsible for spinal cord development. In addition, Wnt-3a plays a regulatory role in autophagy, apoptosis, and regeneration of neurons, neurogenic inflammation and axon regeneration.Wnt-3a promotes the beta-catenin/Tcf pathway which is tumor inducing and can cause cancer when expressed in particular cell populations. It is also one of the most commonly used cytokines for organoid construction.


Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a


[1] Gao K, Zhang T, Wang F, Lv C. Therapeutic Potential of Wnt-3a in Neurological Recovery after Spinal Cord Injury. Eur Neurol. 2019;81(3-4):197-204. doi: 10.1159/000502004. Epub 2019 Jul 23. PMID: 31336380.

[2] Pashirzad M, Fiuji H, Khazei M, Moradi-Binabaj M, Ryzhikov M, Shabani M, Avan A, Hassanian SM. Role of Wnt3a in the pathogenesis of cancer, current status and prospective. Mol Biol Rep. 2019 Oct;46(5):5609-5616. doi: 10.1007/s11033-019-04895-4. Epub 2019 Jun 24. PMID: 31236761.