货号价格

TFN-HM101-100μg / 询价

TFN-HM101-500μg / 询价

TFN-HM101-500μgx2 / 询价

Human Transferrin Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Transferrin Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Val20-Pro698 [Accession | AAH59367].

分子量大小(Molecular Weight)

The protein has a predicted MW of 76.3 kDa. Due to glycosylation, the protein migrates to 78-82 kDa based on Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Transferrin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Transferrin is greater than 95% as determined by SEC-HPLC.

SPR Data

Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 27.50 nM as determined in SPR assay (Biacore T200).

背景(Background)

Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.

分子别名(Synonyms)

Serotransferrin; Transferrin; Siderophilin; TF; PRO1557; PRO2086; HEL-S-71p; TFQTL1

文献(References)

(1) Murakami Y, Saito K, Ito H, Hashimoto Y. Transferrin isoforms in cerebrospinal fluid and their relation to neurological diseases. Proc Jpn Acad Ser B Phys Biol Sci. 2019;95(5):198-210. doi: 10.2183/pjab.95.015. PMID: 31080188; PMCID: PMC6742728.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.