Human TACI/TNFRSF13B Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human TACI/TNFRSF13B Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ser2-Thr166.[Accession | O14836-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 45.3 kDa. Due to glycosylation, the protein migrates to 38-40 kDa and 50-55 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human TACI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human TACI is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human BAFF Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.

背景(Background)

Transmembrane activator and calcium-modulating cyclophilin ligand interactor (TACI) is one of the receptors of B cell activating factor of the tumor necrosis factor family (BAFF) and a proliferation-inducing ligand (APRIL). TACI is a regulator in the immune responses. TACI inhibits B cell expansion and promotes the differentiation and survival of plasma cells.

分子别名(Synonyms)

CD267; TNFRSF13B; TACI; CVID; CVID2; IGAD2; RYZN; TACI

文献(References)

(1) Zhang Y, Li J, Zhang YM, Zhang XM, Tao J. Effect of TACI signaling on humoral immunity and autoimmune diseases. J Immunol Res. 2015;2015:247426. doi: 10.1155/2015/247426. Epub 2015 Mar 17. PMID: 25866827; PMCID: PMC4381970.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.