货号价格

MHC-HM431-100μg / 询价

MHC-HM431-500μg / 询价

MHC-HM431-1mg / 询价

Human WT-1 (HLA-A*02:01) Complex Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human WT-1 (HLA-A*02:01) Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL]

分子量大小(Molecular Weight)

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human WT-1 (HLA-A*02:01) Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human WT-1 (HLA-A*02:01) Complex was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human WT-1 (HLA-A*02:01) Complex, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA.

SPR Data

Human WT-1 (HLA-A*02:01) Complex, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-WT-1 (HLA-1*02:01) Antibody, hFc Tag with an affinity constant of 0.33nM as determined in SPR assay (Biacore T200).

背景(Background)

The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and RMFPNAPYL peptide of  the WT-1.

分子别名(Synonyms)

MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33

文献(References)

(1)Dao T , Pankov D , Scott A , et al. Therapeutic bispecific T-cell engager antibody targeting the intracellular oncoprotein WT1[J]. Nature Biotechnology, 2015.

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