货号价格

MHC-HM402T-100μg / 询价

MHC-HM402T-500μg / 询价

MHC-HM402T-500μgx2 / 询价

Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA]

分子量大小(Molecular Weight)

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).

SPR Data

Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag immobilized on CM5 Chip can bind Tebentafusp with an affinity constant of 0.196 nM as determined in SPR assay (Biacore T200).

背景(Background)

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and YLEPGPVTA peptide of  the gp100.

分子别名(Synonyms)

HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100

文献(References)

(1)Abad J D , Wrzensinski C , Overwijk W , et al. T-Cell Receptor Gene Therapy of Established Tumors in a Murine Melanoma Model[J]. Journal of Immunotherapy, 2008, 31(1):1-6.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.